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pbpX [2018-02-16 12:32:25]
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pbpX [2018-02-16 12:32:25]

penicillin-binding protein X
Locus
BSU16950
Isoelectric point
9.71
Molecular weight
43.70 kDa
Protein length
391 aa Sequence Blast
Gene length
1173 bp Sequence Blast
Function
endopeptidase
Product
penicillin-binding protein X
Essential
no
Synonyms

Genomic Context

      
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Categories containing this gene/protein

Gene

Coordinates
1,765,859 → 1,767,034

Phenotypes of a mutant

  • increased sensitivity to lysozyme PubMed
  • inactivation of pbpX strongly restores beta-lactam resistance in a sigM mutant PubMed
  • The protein

    Localization

  • extracellular (signal peptide) PubMed
  • localizes during sporulation to both asymmetric septa and the prespore PubMed
  • Expression and Regulation

    Operons

    Genes
    Description

    Sigma factors

  • SigM: sigma factor, PubMed, in SigM regulon
  • SigX: sigma factor, PubMed, in SigX regulon
  • SigV: sigma factor, PubMed, in SigV regulon
  • Regulation

  • expression is reduced in a SigV mutant PubMed
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    Biological materials

    Mutant

  • MGNA-B074 (pbpX::erm), available at the NBRP B. subtilis, Japan
  • BKE16950 (ΔpbpX::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTCATTTTTTCCTCCCAA, downstream forward: _UP4_TGATTGGCGATCTTCTTTTG
  • BKK16950 (ΔpbpX::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTCATTTTTTCCTCCCAA, downstream forward: _UP4_TGATTGGCGATCTTCTTTTG
  • GFP fusion

  • 3107 trpC2 pbpX::pSG5047 (cat Pxyl-gfpa-pbpX1-426) PubMed, available in Dirk Jan Scheffers' lab and in the BGSC
  • References

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